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Marco Nooteboom

Marco Nooteboom, CSBB Post-doc since October 2010

WP2: Measuring mitochondrial function at multiple levels of complexity

AL2: Live cell microscopy analysis of mitochondrial function and metabolites

This project focuses on the quantification of metabolite concentrations and metabolite dynamics in living cells. In particular this project will focus on cellular ATP. The aim is to constrain the dynamic mathematical model that will be constructed in WP1. Additionally, together with WP1, the project will focus on the complex role between metabolites to identify potential biochemical pathways as targets for pharmaceutical intervention in WP3. The techniques that will be applied are live cell microscopy along with classic biochemical techniques. Linear unmixing algorithms will be developed to allow simultaneous measurement of multiple fluorescent reporter molecules with overlapping excitation/emission spectra (WP1).

Personal profile: Marco obtained his bachelor degree in Biology and Medical Laboratory Science at Avans Hogeschool in Breda. He performed two internships at the Institute for Medical Technology in Tampere (Finland) under the supervision of Prof. dr. Hans Spelbrink and at the Institute for Ageing and Health in Newcastle (UK) under the supervision of Prof. Doug Turnbull. Both of these placements involved mitochondrial DNA mutations in health and disease. Marco continued in 2007 in the group of Prof. Doug Turnbull as PhD student to further understand the cell-biological consequences of mitochondrial (dys)function in health/ageing and disease. The focus was on tissue examination of colonic crypt stem cells and live-cell microscopy of mitochondrial dynamics in cultured differentiated neurons from embryonic stem cells. He obtained his PhD in mitochondrial biology in 2011 and from there started working as postdoc in the Centre for Systems Biology and Bioenergetics. His research interests are to understand the role of mitochondrial physiology in health and mitochondrial/neurodegenerative diseases. With the use of live-cell fluorescence microscopy he aims to gain more understanding on the relation between mitochondrial morphology and function to determine where and when the delicate balance between these parameters is disrupted in disease.

Recent key CSBB publications:
Breuer ME, Willems PH, Smeitink JAM, Koopman WJ, Nooteboom M. Cellular and animal models for mitochondrial complex I deficiency: A focus on the NDUFS4 subunit. IUBMB Life. 2013 Feb 3. [Epub ahead of print] http://www.ncbi.nlm.nih.gov/pubmed/23378164

Szklarczyk R, Wanschers BFJ, Nijtmans LG, Rodenburg RJ, Zschocke J, Dikow N, van den Brand MAM, Hendriks-Franssen MGM, Gilissen C, Veltman JA, Nooteboom M, Koopman WJH, Willems PHGM, Smeitink JAM, Huynen MA, van den Heuvel LP. A mutation in the FAM36A gene, the human ortholog of COX20, impairs cytochrome c oxidase assembly and is associated with ataxia and muscle hypotonia. Hum Mol Genet. 2012 Nov 2. [Epub ahead of print] http://www.ncbi.nlm.nih.gov/pubmed/23125284

Willems P, Wanschers BF, Esseling J, Szklarczyk R, Kudla U, Duarte I, Forkink M, Nooteboom M, Swarts H, Gloerich J, Nijtmans L, Koopman W, Huynen MA. BOLA1 Is an Aerobic Protein That Prevents Mitochondrial Morphology Changes Induced by Glutathione Depletion. Antioxid Redox Signal. 2012 Sep 11. [Epub ahead of print] http://www.ncbi.nlm.nih.gov/pubmed/22746225

Nooteboom M, Forkink M, Willems PH, Koopman WJ. Live-cell quantification of mitochondrial functional parameters. Visualization Techniques: From Immunohistochemistry to Magnetic Resonance Imaging, Neuromethods Volume 70,2012, Springer Science, DOI: 10.1007/978-1-61779-897-9 http://link.springer.com/protocol/10.1007%2F978-1-61779-897-9_6

Liemburg-Apers DC, Imamura H, Forkink M, Nooteboom M, Swarts HG, Brock R, Smeitink JA, Willems PH, Koopman WJ. Quantitative glucose and ATP sensing in mammalian cells. Review Pharm Res. 2011 Nov;28(11):2745-57 http://www.ncbi.nlm.nih.gov/pubmed/21691894

Contact details:
Centre for Systems Biology & Bioenergetics
Radboud University Nijmegen Medical Centre
Geert Grooteplein 26-28
Huispost 286
6500 HB Nijmegen
The Netherlands
T: +31-24-3613517
Email: m.nooteboom@ncmls.ru.nl