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Herma Renkema

WP2: Measuring mitochondrial function at multiple levels of complexity

AL 6: Analysis of fluxomics

The following methods have been set-up / adapted to determine mitochondrial energy metabolism: oxidation assays using 14C labeled substrates, ATP and creatine-phosphate production measurements, and quantification of metabolites in cell culture media (glucose, pyruvate, lactate, organic- and amino-acids). Currently these methods are being applied to study mitochondrial energy metabolism in patient fibroblast cell lines and myoblasts and myofibers of complex I-deficient KO-mice, although the latter is being hampered by breeding problems. Furthermore, a pipeline for lentiviral complementation of cell lines, which is used to generate immortalized patient myoblasts, has been set up. This system will also be used for the introduction of reporter genes, and to complement genetic defects in patient cell lines, in order to serve as control samples with identical genetic background as mutated patient cell lines.

Herma Renkema has obtained her PhD in Biochemistry at the University of Amsterdam in 1997. She has worked as a Post-doctoral fellow and junior-group leader in several laboratories on subjects involving biochemistry and protein-protein interactions. Within the CSBB her responsibilities are to develop quantitative assays required for testing the in silico predictions of the genome scale model. Furthermore she will implement a system for lentiviral transduction of patient cell lines.

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