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Jori Joosten

Jori Joosten-Wagenaars, Technician since Januari 2012,

WP2: Measuring mitochondrial function at multiple levels of complexity

AL2: Live cell mitochondria and single cells

This action line aims to use live-cell microscopy to quantitatively measure relevant parameters for mitochondrial and cellular fitness. These parameters will include mitochondrial membrane
potential, mitochondrial matrix pH, free cytosolic and mitochondrial [ATP] and cytosolic glucose using novel reporter proteins, reactive oxygen species (ROS), and cytosolic and mitochondrial Ca2+ handling. Obtained results will lead to life cell constraints for the flux and dynamic model(s) developed in WP1. Finally, we will analyze the time evolution of the above readouts and the effect of the diseased state and/or interventions thereupon.

Jori Joosten-Wagenaars obtained her bachelor in Zoology at the Hogeschool Utrecht in June 2007. She worked for 4,5 years in the field of cancer research/immunology at the department of Tumorimmunology at the UMC Nijmegen, where she specialized herself in animal experimentation. She is currently working as a research technician for the CSBB where she isolates individual  muscle fibers and muscle cells for the analysis of relevant mitochondrial parameters in healthy and diseased myotubes. Furthermore, she has the responsibility for the genotyping of the mouse breeding line and also assists several Postdoc’s and PhD students in their work.

Email: j.wagenaars@ncmls.ru.nl